MLYCD Polyklonaler Antikörper

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• KD/KO Validated

MLYCD Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 15265-1-AP

Synonyme

malonyl CoA decarboxylase, MCD, MLYCD

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Galerie der Validierungsdaten

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  WB analysis of human testis using 15265-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15265-1-AP (MLYCD antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15265-1-AP

  IP result of anti-MLYCD(IP:15265-1-AP, 4ug; Detection:15265-1-AP 1:1000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human kidney using 15265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15265-1-AP (MLYCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15265-1-AP (MLYCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15265-1-AP bindet in WB, IP, IHC, IF, ELISA MLYCD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLYCD fusion protein Ag7334
• Vollständiger Name: malonyl-CoA decarboxylase
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC000286
• Gene symbol: MLYCD
• Gene ID (NCBI): 23417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLYCD, also named as MCD, is a 50-55 kDa protein. It is an enzyme that catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. 66 kDa MLYCD protein has been identified in normal cell lines. Some patients give the 68-83 kDa smeary band. (PMID:12955715)

Publikationen

Species	Application	Title
human	WB	Hepatology; Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.; Authors - Ming-Da Wang
mouse	WB	Proc Natl Acad Sci U S A; Ribosomal protein-Mdm2-p53 pathway coordinates nutrient stress with lipid metabolism by regulating MCD and promoting fatty acid oxidation.; Authors - Yong Liu
mouse	WB	Cell Rep; Acetyl-CoA carboxylase 1 is a suppressor of the adipocyte thermogenic program; Authors - Adilson Guilherme ; KO Validated
		PLoS Pathog; Integrated systems biology analysis of KSHV latent infection reveals viral induction and reliance on peroxisome mediated lipid metabolism.; Authors - Zoi E Sychev
rat	WB	Int J Mol Sci; The Impact of Nicotine along with Oral Contraceptive Exposure on Brain Fatty Acid Metabolism in Female Rats; Authors - Shahil H Patel
mouse	WB	J Mol Cell Cardiol; C-Myc induced compensated cardiac hypertrophy increases free fatty acid utilization for the citric acid cycle.; Authors - Olson Aaron K AK

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