MZB1 Polyklonaler Antikörper

MZB1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11454-1-AP

Synonyme

hypothetical protein MGC29506, MZB1, PACAP, pERp1

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Galerie der Validierungsdaten

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  WB analysis of Raji using 11454-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11454-1-AP (MZB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11454-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11454-1-AP (MZB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11454-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11454-1-AP (MZB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 11454-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 11454-1-AP (MZB1 Antibody) at dilution of 1:50.

• 

  IHC staining of human small intestine using 11454-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 11454-1-AP (MZB1 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Produktinformation

11454-1-AP bindet in WB, IF, IHC, ELISA MZB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MZB1 fusion protein Ag2015
• Vollständiger Name: hypothetical protein MGC29506
• Berechnetes Molekulargewicht: 189 aa, 21 kDa
• Beobachtetes Molekulargewicht: 19-21 kDa
• GenBank-Zugangsnummer: BC021275
• Gene symbol: MZB1
• Gene ID (NCBI): 51237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.

Publikationen

Species	Application	Title
human	IHC	Clin Cancer Res; Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor suppressor in hepatocellular carcinoma.; Authors - Matsumura Satoshi S
human	IHC,IF	J Pathol; Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney.; Authors - Huiya Xu
human	IHC	Int J Cancer; Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer.; Authors - Mitsuro Kanda
human	WB	J Biol Chem; Transgenic expression of microRNA-185 causes a developmental arrest of T cells by targeting multiple genes including Mzb1.; Authors - Belkaya Serkan S
human	WB,IHC,IF	Arthritis Res Ther; Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes.; Authors - Aya Miyagawa-Hayashino
human	IHC	Oncol Lett; MZB1 expression indicates poor prognosis in estrogen receptor-positive breast cancer.; Authors - Manabu Watanabe

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