AFP Polyclonal antibody

AFP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

115

Cat no : 14550-1-AP

Synonyms

HPAFP, Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein

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Validation Data Gallery

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  WB analysis using 14550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14550-1-AP

  WB result of AFP antibody (14550-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells.

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  WB analysis of BxPC-3 using 14550-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14550-1-AP

  IP result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 14550-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14550-1-AP (AFP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HepG2 cells, HuH-7 cells, L02 cells, HepG2cells, human placenta tissue
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 32 publications below
• IHC: See 46 publications below
• IF: See 41 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

14550-1-AP targets AFP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFP fusion protein Ag6089
• Full Name: alpha-fetoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC027881
• Gene Symbol: AFP
• Gene ID (NCBI): 174
• RRID: AB_2223933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

Publications

Species	Application	Title
mouse	IHC	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	IHC	Cancer Cell; Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.; Authors - Ruidong Xue
mouse	IF	Nat Commun; ATAXIN-2 intermediate-length polyglutamine expansions elicit ALS-associated metabolic and immune phenotypes; Authors - Renata Vieira de Sá
mouse	IHC	J Clin Invest; Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.; Authors - Xueru Mu
human	IHC	J Clin Invest; HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis.; Authors - Celine Hernandez
human,mouse	WB,IHC,IF	Nat Commun; Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.; Authors - Wei Cheng

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