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  • Featured Product
  • KD/KO Validated

c-Met (N-terminal) Polyklonaler Antikörper

c-Met (N-terminal) Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19971-1-AP

Synonyme

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of HeLa cells using c-Met (N-terminal) Polyclonal antibody (19971-1-AP)

    WB analysis of HeLa using 19971-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19971-1-AP (c-Met (N-terminal) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using c-Met (N-terminal) Polyclonal antibody (19971-1-AP)

    IHC staining of human breast cancer using 19971-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19971-1-AP (c-Met (N-terminal) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using c-Met (N-terminal) Polyclonal antibody (19971-1-AP)

    IHC staining of human breast cancer using 19971-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19971-1-AP (c-Met (N-terminal) antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using c-Met (N-terminal) Polyclonal antibody (19971-1-AP)

    IHC staining of human colon using 19971-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19971-1-AP (c-Met (N-terminal) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using c-Met (N-terminal) Polyclonal antibody (19971-1-AP)

    IHC staining of human colon using 19971-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19971-1-AP (c-Met (N-terminal) antibody at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HeLa cells using c-Met (N-terminal) Polyclonal antibody (19971-1-AP)

    FC experiment of HeLa using 19971-1-AP

    1X10^6 HeLa cells were stained with 0.2ug c-Met (N-terminal) antibody (19971-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:200
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below
IHCSee 1 publications below

Produktinformation

19971-1-AP bindet in WB, IHC, FC,ELISA c-Met (N-terminal) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name met proto-oncogene (hepatocyte growth factor receptor)
Berechnetes Molekulargewicht 156 kDa
Beobachtetes Molekulargewicht 140 kDa, 50 kDa
GenBank-ZugangsnummerNM_000245
Gene symbol MET
Gene ID (NCBI) 4233
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). This antibody recognizes the N-term of c-Met.

Publikationen

SpeciesApplicationTitle
humanWB

Oncogene

A regulatory circuit composed of DNA methyltransferases and receptor tyrosine kinases controls lung cancer cell aggressiveness.

Authors - F Yan
View Article
humanWB

Carcinogenesis

MicroRNA-34a inhibits migration and invasion of colon cancer cells via targeting to Fra-1.

Authors - Wu Jianmin J
View Article
mouseIHC

Am J Pathol

PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer.

Authors - Wen-Cheng Chung
View Article