LIN28 Polyklonaler Antikörper

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• KD/KO Validated

LIN28 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

51

Kat-Nr. : 11724-1-AP

Synonyme

Protein lin 28 homolog A, Lin-28A, LIN28A, Lin 28A, LIN 28

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Galerie der Validierungsdaten

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  WB analysis using 11724-1-AP

  Various lysate were subjected to SDS PAGE followed by western blot with 11724-1-AP (LIN28 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT cells using 11724-1-AP

  WB data of LIN28 antibody (11724-1-AP, 1:500) with si-control and si-LIN28 transfected NCCIT cells.

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  IP experiment of K-562 using 11724-1-AP

  IP result of anti-LIN28 (IP:11724-1-AP, 4ug; Detection:11724-1-AP 1:2000) with K-562 cells lysate 1360 ug.

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  IHC staining of mouse testis using 11724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11724-1-AP (LIN28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11724-1-AP (LIN28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human embronic stem cells using 11724-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (10X).

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  IF Staining of human embronic stem cells using 11724-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Mausembryogewebe, NCCIT-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: humane embryonale Stammzellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 27 publications below
• IHC: See 18 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

11724-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LIN28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIN28 fusion protein Ag2312
• Vollständiger Name: lin-28 homolog (C. elegans)
• Berechnetes Molekulargewicht: 209 aa, 23 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC028566
• Gene symbol: LIN28
• Gene ID (NCBI): 79727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Publikationen

Species	Application	Title
mouse	IHC	Nature; A role for Lin28 in primordial germ-cell development and germ-cell malignancy.; Authors - West Jason A JA
human,mouse	WB,IHC	Nat Genet; Lin28 promotes transformation and is associated with advanced human malignancies.; Authors - Viswanathan Srinivas R SR
mouse	IHC	Nat Genet; Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies.; Authors - Zhu Hao H
human	WB,IF	Nat Methods; A nonviral minicircle vector for deriving human iPS cells.; Authors - Jia Fangjun F
human	IF	Cell Stem Cell; Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.; Authors - Carmen Lorenz
mouse	IHC	Cell Res; Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis.; Authors - Phillip J Hsu

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