ZCCHC11 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZCCHC11 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 18980-1-AP

Synonyms

TUTase 4, TUT4, Terminal uridylyltransferase 4, PAPD3, KIAA0191

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Validation Data Gallery

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  WB analysis of MCF-7 using 18980-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18980-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 18980-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18980-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18980-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 18980-1-AP

  IP result of anti-ZCCHC11 (IP:18980-1-AP, 5ug; Detection:18980-1-AP 1:1000) with MCF-7 cells lysate 2000ug.

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  IF Staining of HepG2 using 18980-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18980-1-AP (ZCCHC11 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 23 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

18980-1-AP targets ZCCHC11 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: zinc finger, CCHC domain containing 11
• Calculated Molecular Weight: 185 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: NM_015269
• Gene Symbol: ZCCHC11
• Gene ID (NCBI): 23318
• RRID: AB_10598327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.

Publications

Species	Application	Title
mouse	WB	Nature; mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.; Authors - Marcos Morgan ; KO Validated
mouse	WB	Nature; A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.; Authors - Chang Hao-Ming HM ; KD Validated
human	WB	Cell; Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.; Authors - Piskounova Elena E ; KD Validated
human	WB,IF	Cell; Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.; Authors - Zbigniew Warkocki
human	WB	Mol Cell; 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.; Authors - Acong Yang ; KO Validated
human	WB	Mol Cell; A Mechanism for microRNA Arm Switching Regulated by Uridylation.; Authors - Haedong Kim ; KD Validated

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