Validation Data Gallery
|Positive IHC detected in
|human colon cancer tissue, human brain tissue, human spleen tissue, human testis tissue, human kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|IHC : 1:50-1:500
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16178-1-AP targets Lin28B-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|lin-28 homolog B (C. elegans)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.
Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.
Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.
LOC101929709 promotes gastric cancer progression by aiding LIN28B to stabilize c-MYC mRNA
Testicular expression of the Lin28/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty.
Am J Cancer Res
Cellular 5-hydroxylmethylcytosine content determines tumorigenic potential and prognosis of pancreatic ductal adenocarcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (01-24-2023)
Antibody worked well