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  • KD/KO Validated

Lin28B-specific Polyclonal antibody

Lin28B-specific Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16178-1-AP

Synonyms

CSDD2, lin 28 homolog B (C. elegans), Lin 28B, Lin28, LIN28B, Lin28B-specific, Protein lin 28 homolog B



Tested Applications

Positive IHC detected inhuman colon cancer tissue, human brain tissue, human spleen tissue, human testis tissue, human kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16178-1-AP targets Lin28B-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lin-28 homolog B (C. elegans)
Calculated molecular weight 28 kDa
Observed molecular weight27 kDa
GenBank accession numberNM_001004317
Gene symbol LIN28B
Gene ID (NCBI) 389421
RRIDAB_2135051
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.

Protocols

Product Specific Protocols
IHC protocol for Lin28B-specific antibody 16178-1-APDownload protocol
IF protocol for Lin28B-specific antibody 16178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.

Authors - Domenico Albino
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
  • KD Validated
humanIHC

Gastric Cancer

LOC101929709 promotes gastric cancer progression by aiding LIN28B to stabilize c-MYC mRNA

Authors - Tong-Peng Xu
humanWB

Mol Ther

PMID: 29456019

Authors - Xinyi Wang
ratIHC

Sci Rep

Testicular expression of the Lin28/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty.

Authors - S Sangiao-Alvarellos
humanIHC

Am J Cancer Res

Cellular 5-hydroxylmethylcytosine content determines tumorigenic potential and prognosis of pancreatic ductal adenocarcinoma.

Authors - Yi-Lng Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (01-24-2023)

Antibody worked well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A172