LGALS3BP Polyklonaler Antikörper

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• KD/KO Validated

LGALS3BP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 10281-1-AP

Synonyme

M2BP, Lectin galactoside-binding soluble 3-binding protein, Galectin-3-binding protein, Galectin 3 binding protein, BTBD17B

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10281-1-AP

  WB result of LGALS3BP antibody (10281-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LGALS3BP transfected HepG2 cells.

• 

  WB analysis using 10281-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10281-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10281-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10281-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10281-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10281-1-AP

  IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

• 

  IP experiment of HEK-293 using 10281-1-AP

  IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:400) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10281-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LGALS3BP antibody (10281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, COLO 320-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HepG2-Zellen, humanes Milch-Gewebe, humanes Plasmagewebe
• Erfolgreiche IP: HepG2-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

10281-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LGALS3BP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGALS3BP fusion protein Ag0294
• Vollständiger Name: lectin, galactoside-binding, soluble, 3 binding protein
• Berechnetes Molekulargewicht: 585 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-90 kDa
• GenBank-Zugangsnummer: BC002403
• Gene symbol: LGALS3BP
• Gene ID (NCBI): 3959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	IHC	Cancer Res; A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.; Authors - Silverman Ayaka M AM ; KD Validated
human	IF	Blood Adv; Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.; Authors - Yu Akahoshi
mouse	WB	PLoS Pathog; Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.; Authors - Gang Xu
human	WB	Thromb Haemost; Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles.; Authors - Smalley David M DM
human	IHC	Cancer Sci; MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain.; Authors - Huanxi Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Beautiful antibody - produces a single band at the correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LNCaP

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