LC3A/B Monoklonaler Antikörper
LC3A/B Monoklonal Antikörper für ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus und mehr (2)
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
2B2B5
Kat-Nr. : 66139-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 16 publications below |
| IHC | See 1 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Produktinformation
66139-1-Ig bindet in WB, IP, IHC, IF, ELISA LC3A/B und zeigt Reaktivität mit Hausschwein, human, Maus
| Getestete Reaktivität | Hausschwein, human, Maus |
| In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Fusionsprotein |
| Vollständiger Name | microtubule-associated protein 1 light chain 3 alpha |
| Berechnetes Molekulargewicht | 14 kDa |
| Beobachtetes Molekulargewicht | 14 kDa, 16 kDa |
| GenBank-Zugangsnummer | NM_032514 |
| Gene symbol | MAP1LC3A |
| Gene ID (NCBI) | 84557 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Cell Death Dis Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy. | ||
Cell Prolif S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos. | ||
Mol Neurobiol Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy. | ||
Aging (Albany NY) Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs. | ||
Front Mol Neurosci Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models. |
