KDM3A,JMJD1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KDM3A,JMJD1A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

41

Kat-Nr. : 12835-1-AP

Synonyme

KDM3A, JMJD1, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 12835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12835-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12835-1-AP

  IP result of anti-KDM3A,JMJD1A (IP:12835-1-AP, 5ug; Detection:12835-1-AP 1:1000) with HeLa cells lysate 2500ug.

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  IHC staining of human ovary tumor using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 12835-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12835-1-AP

  Immunofluorescent analysis of HepG2 cells, using KDM3A antibody 12835-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Eierstockgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 35 publications below
• IHC: See 10 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 6 publications below

Produktinformation

12835-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA KDM3A,JMJD1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KDM3A,JMJD1A fusion protein Ag3581
• Vollständiger Name: lysine (K)-specific demethylase 3A
• Berechnetes Molekulargewicht: 150 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC038297
• Gene symbol: KDM3A/JMJD1A
• Gene ID (NCBI): 55818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC. (PMID:20823141) This antibody recognize the N-term of KDM3A(JMJD1A).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Nat Commun; Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.; Authors - Riina Kaukonen ; KD Validated
human	WB	Nat Commun; Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.; Authors - Valérie Brügger
human	WB	Biomaterials; Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer.; Authors - Chao He
mouse	WB	EMBO J; Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.; Authors - Khoa A Tran
human	WB	Proc Natl Acad Sci U S A; Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.; Authors - Lingling Fan ; KD Validated
mouse	WB,IF	J Cell Biol; KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.; Authors - Patricia L Yeyati ; KO Validated

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