KDM3B Polyclonal antibody

KDM3B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19915-1-AP

Synonyms

JmjC domain-containing histone demethylation protein 2B, JHDM2B, EC:1.14.11.65, C5orf7, 5qNCA

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Validation Data Gallery

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  WB analysis using 19915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19915-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 19915-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KDM3B antibody (19915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19915-1-AP targets KDM3B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDM3B fusion protein Ag13795
• Full Name: lysine (K)-specific demethylase 3B
• Calculated Molecular Weight: 1761 aa, 192 kDa
• Observed Molecular Weight: 192 kDa, 85 kDa
• GenBank Accession Number: BC001202
• Gene Symbol: KDM3B
• Gene ID (NCBI): 51780
• RRID: AB_10693529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Genet; N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.; Authors - Yuan Li