MKL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MKL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

32

Reviews (1)

Cat no : 21166-1-AP

Synonyms

Myocardin-related transcription factor A, MRTF-A, MRTFA, MKL/myocardin-like protein 1, MAL

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Validation Data Gallery

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  WB analysis using 21166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21166-1-AP (MKL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 21166-1-AP

  Immunofluorescent analysis of HeLa cells using 21166-1-AP (MKL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human small intestine tissue, human tonsillitis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• ChIP: See 4 publications below

Product Information

21166-1-AP targets MKL1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKL1 fusion protein Ag15240
• Full Name: megakaryoblastic leukemia (translocation) 1
• Calculated Molecular Weight: 931 aa, 99 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC115039
• Gene Symbol: MKL1
• Gene ID (NCBI): 57591
• RRID: AB_2878822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Stem Cell; An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.; Authors - Allison P Kann
mouse	IF	Nat Cell Biol; Myc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation; Authors - Miguel Bernabé-Rubio
	IF	J Cell Biol; The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression.; Authors - Jaime Fernández-Barrera
mouse	IF	Elife; Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors.; Authors - Colin J Dinsmore
human	WB,IHC,IF,ChIP	Exp Mol Med; MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β; Authors - Fu Du ; KD Validated
human	WB	Oncotarget; The Wnt-β-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells.; Authors - Hongpeng He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Qingxue (Verified Customer) (06-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs