TIP60/KAT5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TIP60/KAT5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 10827-1-AP

Synonyme

60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10827-1-AP

  WB result of KAT5 antibody (10827-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAT5 transfected HepG2 cells.

• 

  WB analysis of PC-3 using 10827-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10827-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10827-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10827-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10827-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10827-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10827-1-AP (KAT5 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

10827-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA TIP60/KAT5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIP60/KAT5 fusion protein Ag1261
• Vollständiger Name: K(lysine) acetyltransferase 5
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 70 kDa
• GenBank-Zugangsnummer: BC000166
• Gene symbol: KAT5
• Gene ID (NCBI): 10524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa

Publikationen

Species	Application	Title
human	WB	Nature; ANP32E is a histone chaperone that removes H2A.Z from chromatin.; Authors - Arnaud Obri
mouse	WB	Nat Immunol; The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells.; Authors - Jing Geng
		Mol Cell; PMID: 28366643; Authors - Sophie Barral
human	WB	Nat Commun; Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.; Authors - Zhiyuan You ; KD Validated
human	IF,IP	J Mol Cell Biol; Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis; Authors - Hameed Akbar
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu

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