IGFBP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IGFBP3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Rezensionen (1)

Kat-Nr. : 10189-2-AP

Synonyme

IGF-binding protein 3, IGF binding protein 3, IBP-3, IBP3, IBP 3

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Galerie der Validierungsdaten

• 

  WB analysis of mouse colon using 10189-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10189-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10189-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10189-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10189-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 10189-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, A2780-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 7 publications below
• IF: See 8 publications below

Produktinformation

10189-2-AP bindet in WB, IHC, IF/ICC, ELISA IGFBP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGFBP3 fusion protein Ag0242
• Vollständiger Name: insulin-like growth factor binding protein 3
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32-37 kDa
• GenBank-Zugangsnummer: BC000013
• Gene symbol: IGFBP3
• Gene ID (NCBI): 3486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

Publikationen

Species	Application	Title
human	IF	Cell; 3D reconstruction of a gastrulating human embryo; Authors - Zhenyu Xiao
human,mouse	WB,IHC,IF	Theranostics; Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p; Authors - Soo Hyun Kang ; KD Validated
		Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
mouse	WB	Acta Physiol (Oxf); Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.; Authors - Yingying Zhang
human	WB	Cancer Lett; Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.; Authors - Yuan Yin
mouse	WB	PLoS Biol; The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.; Authors - Patrick A Carroll

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Margarita (Verified Customer) (04-19-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: PC3 cells

IGFBP3 Antibody Immunofluorescence validation (1:100 dilution) in PC3 cells (Cat no:10189-2-AP)

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