HSP60 Monoklonaler Antikörper

HSP60 Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

34

CloneNo.

2F10E7

Kat-Nr. : 66041-1-Ig

Synonyme

60 kDa chaperonin, Chaperonin 60, GROEL, Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1

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Galerie der Validierungsdaten

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  WB analysis using 66041-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IP experiment of mouse liver using 66041-1-Ig

  IP Result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human spleen using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66041-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

• 

  IF Staining of Neuro-2a using 66041-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66041-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug HSP60 antibody (66041-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Hautkrebsgewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

66041-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA HSP60 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hefe, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP60 fusion protein Ag7848
• Vollständiger Name: heat shock 60kDa protein 1 (chaperonin)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC003030
• Gene symbol: HSPD1
• Gene ID (NCBI): 3329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Publikationen

Species	Application	Title
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang
human	WB, IF	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
human	WB	Autophagy; Substitution of PINK1 Gly411 modulates substrate receptivity and turnover; Authors - Fabienne C Fiesel
mouse	WB	J Clin Invest; ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage/cGAS-STING activation.; Authors - Mengjie Hu
mouse	IF	Autophagy; Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage.; Authors - Yuhua Zhang
mouse	WB	Autophagy; Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.; Authors - Yinjuan Song

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