GATA4-Specific Polyklonaler Antikörper

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• KD/KO Validated

GATA4-Specific Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Kat-Nr. : 19530-1-AP

Synonyme

GATA binding factor 4, GATA binding protein 4, GATA4, GATA4-Specific, Transcription factor GATA 4

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 19530-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19530-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 19530-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausherzgewebe, Mauslebergewebe, NIH/3T3-Zellen, PC-3-Zellen, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

19530-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA GATA4-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GATA binding protein 4
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_002052
• Gene symbol: GATA4
• Gene ID (NCBI): 2626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of GATA4? Is GATA4 post-translationally modified?

The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).

 What is the subcellular localization of GATA4?

GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).

 What is the tissue expression pattern of GATA4?

GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608).

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB,IHC,IF,IP	Clin Transl Med; Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.; Authors - Haiyun Chen
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang
human	WB	Cardiovasc Res; Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR26b-5p and miR-140-3p binding to Gata4.; Authors - Hui Li
mouse	WB	J Bone Miner Res; Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription; Authors - Qiuyi Wang
mouse	WB	Cell Prolif; Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.; Authors - Li Chen

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