Kindlin 2 Polyklonaler Antikörper

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• KD/KO Validated

Kindlin 2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Rezensionen (1)

Kat-Nr. : 11453-1-AP

Synonyme

Fermitin family homolog 2, FERMT2, KIND2, Kindlin 2, mig 2, MIG2, PLEKHC1, UNC112, UNC112B

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Galerie der Validierungsdaten

• 

  WB analysis of A549 using 11453-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11453-1-AP (Kindlin 2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11453-1-AP

  WB result of Kindlin 2 antibody (11453-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Kindlin 2 transfected A549 cells.

• 

  IP experiment of A549 using 11453-1-AP

  IP result of anti-Kindlin 2 (IP:11453-1-AP, 3ug; Detection:11453-1-AP 1:1000) with A549 cells lysate 3300ug.

• 

  IHC staining of human lung cancer using 11453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11453-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11453-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 27 publications below
• IHC: See 9 publications below
• IF: See 11 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

11453-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA Kindlin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kindlin 2 fusion protein Ag2013
• Vollständiger Name: fermitin family homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 680 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC017327
• Gene symbol: Kindlin 2
• Gene ID (NCBI): 10979
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

Publikationen

Species	Application	Title
human	WB,IP	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo
mouse, zebrafish	WB, IF	Circ Res; Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.; Authors - James J Dowling ; KD Validated
zebrafish	IF	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
mouse,human	WB,IP	J Am Soc Nephrol; Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.; Authors - Ying Sun ; KD Validated
human	WB, IF	J Cell Sci; Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.; Authors - Clotilde Huet-Calderwood ; KD Validated
human	WB	J Cell Sci; Kindlin-2 promotes rear focal adhesion disassembly and directional persistence in cell migration; Authors - Jie Liu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yasmin (Verified Customer) (08-01-2019)

Excellent antibody, highly recommended for western blot.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for western blot, 1:100 for IF
• Cell Tissue Type: HeLa, NIH3T3

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