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  • KD/KO Validated

TCF12/HEB Polyclonal antibody

TCF12/HEB Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14419-1-AP


bHLHb20, DNA binding protein HTF4, E box binding protein, HEB, HsT17266, HTF4, TCF 12, TCF12, transcription factor 12, Transcription factor HTF 4

Tested Applications

Positive WB detected inHepG2 cells, Raji cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14419-1-AP targets TCF12/HEB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCF12/HEB fusion protein Ag5765
Full Name transcription factor 12
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC050556
Gene symbol TCF12
Gene ID (NCBI) 6938
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].


Product Specific Protocols
WB protocol for TCF12/HEB antibody 14419-1-APDownload protocol
IHC protocol for TCF12/HEB antibody 14419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

TCF12 is mutated in anaplastic oligodendroglioma.

Authors - Karim Labreche


A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis.

Authors - Sisu Han

Stem Cells

Tcf12, a Member of Basic Helix-loop-helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation in vitro and in vivo.

Authors - Siqi Yi
  • KD Validated


Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.

Authors - Holly Holliday

BMC Genomics

Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis.

Authors - Thorsen Kasper K

PLoS One

Extensive gene-specific translational reprogramming in a model of B cell differentiation and Abl-dependent transformation.

Authors - Bates Jamie G JG