CLIC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CLIC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12298-2-AP

Synonyms

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

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Validation Data Gallery

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  WB analysis of HeLa using 12298-2-AP

  WB result of CLIC4 antibody (12298-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

• 

  WB analysis of mouse kidney using 12298-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12298-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12298-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 12298-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12298-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, HeLa cells, mouse ovary tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12298-2-AP targets CLIC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLIC4 fusion protein Ag2942
• Full Name: chloride intracellular channel 4
• Calculated Molecular Weight: 253 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC012444
• Gene Symbol: CLIC4
• Gene ID (NCBI): 25932
• RRID: AB_2081969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

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