Kindlin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Kindlin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 22215-1-AP

Synonyms

FERMT1, KIND1, Kindlerin, Kindlin 1, Kindlin1, Kindlin-1, UNC112A, Unc-112-related protein 1, URP1, C20orf42, DTGCU2

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Validation Data Gallery

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  WB analysis using 22215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22215-1-AP (Kindlin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22215-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22215-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22215-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 22215-1-AP

  Immunofluorescent analysis of HEK-293 cells using 22215-1-AP (FERMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, COLO 320 cells, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human pancreas tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

22215-1-AP targets Kindlin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kindlin 1 fusion protein Ag17543
• Full Name: fermitin family homolog 1 (Drosophila)
• Calculated Molecular Weight: 677 aa, 77 kDa
• Observed Molecular Weight: 70-77 kDa
• GenBank Accession Number: BC035882
• Gene Symbol: Kindlin 1
• Gene ID (NCBI): 55612
• RRID: AB_2879033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Int; FERMT1 contributes to the migration and invasion of nasopharyngeal carcinoma through epithelial-mesenchymal transition and cell cycle arrest.; Authors - Lingling Li ; KD Validated
rat	WB	Eur J Neurosci; Wnt10a/β-catenin signaling is involved in kindlin-1-mediated astrocyte activation in a chronic construction injury rat model.; Authors - Baisong Zhao
mouse	IHC,IF	Int J Mol Med; Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing.; Authors - Congcong Shen
mouse,rat	WB,IF	Exp Anim; FERMT1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLRP3/NF-κB pathway; Authors - Jianqiang Wei ; KD Validated
mouse	WB	Cell Rep; Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow; Authors - Yuji Kamioka