EXOC5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EXOC5 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 17593-1-AP

Synonyme

EXOC5, exocyst complex component 5, HSEC10, PRO1912, SEC10, SEC10L1, SEC10P

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 17593-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17593-1-AP (EXOC5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 17593-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 17593-1-AP (EXOC5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17593-1-AP

  IP Result of anti-EXOC5 (IP:17593-1-AP, 3ug; Detection:17593-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human testis using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17593-1-AP

  Immunofluorescent analysis of HepG2 cells, using EXOC5 antibody 17593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Nebennierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

17593-1-AP bindet in WB, IP, IHC, IF, ELISA EXOC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOC5 fusion protein Ag11524
• Vollständiger Name: exocyst complex component 5
• Berechnetes Molekulargewicht: 708 aa, 82 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC041126
• Gene symbol: EXOC5
• Gene ID (NCBI): 10640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.

Publikationen

Species	Application	Title
human	WB	Cell; RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly.; Authors - Bodemann Brian O BO ; KD Validated
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao
human	WB	Sci Rep; Insights into the ubiquitin-proteasome system of human embryonic stem cells.; Authors - Isabel Saez
human	WB,IHC	Mol Cancer Res; EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.; Authors - Haojie Li ; KD Validated
mouse	IF	Cells; The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells; Authors - Nicole K. Nakamura
mouse	WB	J Biol Chem; Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model.; Authors - Brent A Fujimoto ; KO Validated

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