EXOC6B Polyclonal antibody

EXOC6B Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 55440-1-AP

Synonyms

EXOC6B, exocyst complex component 6B, KIAA0919, SEC15 like protein 2, SEC15B, SEC15L2

---

Validation Data Gallery

• 

  IP experiment of HEK-293 using 55440-1-AP

  IP result of anti-EXOC6B (IP:55440-1-AP, 4ug; Detection:55440-1-AP 1:500) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human liver using 55440-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 55440-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 55440-1-AP

  Immunofluorescent analysis of HEK-293 cells using 55440-1-AP (EXOC6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55440-1-AP targets EXOC6B in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: exocyst complex component 6B
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: NM_015189
• Gene Symbol: EXOC6B
• Gene ID (NCBI): 23233
• RRID: AB_2881333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.