ERP29 Polyklonaler Antikörper

ERP29 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 24344-1-AP

Synonyme

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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Galerie der Validierungsdaten

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  WB analysis using 24344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 24344-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24344-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24344-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24344-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 24344-1-AP

  IP Result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 24344-1-AP

  Immunofluorescent analysis of HeLa cells using 24344-1-AP (ERP29 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of human liver cancer using 24344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 24344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HEK-293-Zellen, Mauslebergewebe, Mauslungengewebe, Mausnierengewebe, Rattenhodengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24344-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ERP29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERP29 fusion protein Ag19451
• Vollständiger Name: endoplasmic reticulum protein 29
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC101495
• Gene symbol: ERP29
• Gene ID (NCBI): 10961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).

Publikationen

Species	Application	Title
mouse	WB	iScience; Non-canonical function of DPP4 promotes cognitive impairment through ERp29-associated mitochondrial calcium overload in diabetes; Authors - Jiaxiu Li
mouse	WB,IF,CoIP	Metabolism; Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization; Authors - Ya Hui

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