EIF3M Polyklonaler Antikörper

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• KD/KO Validated

EIF3M Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11423-1-AP

Synonyme

PCID1, hFL-B5, HFLB5, hfl B5, GA17

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Galerie der Validierungsdaten

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  WB analysis using 11423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11423-1-AP (EIF3M antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11423-1-AP

  IP result of anti-EIF3M (IP:11423-1-AP, 4ug; Detection:11423-1-AP 1:2000) with HeLa cells lysate 1080 ug.

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  IHC staining of human pancreas cancer using 11423-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11423-1-AP (EIF3M antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11423-1-AP

  Immunofluorescent analysis of HepG2 cells, using EIF3M antibody 11423-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11423-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA EIF3M und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3M fusion protein Ag1994
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit M
• Berechnetes Molekulargewicht: 374 aa, 43 kDa
• Beobachtetes Molekulargewicht: 35-43 kDa
• GenBank-Zugangsnummer: BC019103
• Gene symbol: EIF3M
• Gene ID (NCBI): 10480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.

Publikationen

Species	Application	Title
human	WB	Nature; Genome-wide silencing in Drosophila captures conserved apoptotic effectors.; Authors - Chew Su Kit SK
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
human	WB,IHC	Oncogene; eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer.; Authors - Goh S-H SH ; KD Validated
human	IF,CoIP	Am J Cancer Res; EIF3m promotes the malignant phenotype of lung adenocarcinoma by the up-regulation of oncogene CAPRIN1.; Authors - Xinwei Liu ; KD Validated
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi

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