EIF3I Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF3I Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 11287-1-AP

Synonyms

Eukaryotic translation initiation factor 3 subunit 2, EIF3S2, eIF-3-beta, eIF3 p36, eIF3 beta

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 11287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11287-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11287-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11287-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11287-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11287-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11287-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11287-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11287-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11287-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11287-1-AP

  IP result of anti-EIF3I (IP:11287-1-AP, 3ug; Detection:11287-1-AP 1:1000) with HEK-293 cells lysate 1500ug.

• 

  IHC staining of human breast cancer using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11287-1-AP (EIF3I antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11287-1-AP (EIF3I antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11287-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11287-1-AP

  Immunofluorescent analysis of Hela cells, using EIF3I antibody 11287-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells, human placenta tissue, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

11287-1-AP targets EIF3I in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3I fusion protein Ag1847
• Full Name: eukaryotic translation initiation factor 3, subunit I
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000413
• Gene Symbol: EIF3I
• Gene ID (NCBI): 8668
• RRID: AB_2097068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.

Publications

Species	Application	Title
human	WB	Metab Eng; Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis.; Authors - Anne Roobol
rat	IHC,WB	J Bone Miner Res; TRIP-1: a regulator of osteoblast function.; Authors - Metz-Estrella Diana D ; KD Validated
mouse, hamster	WB	Biochem J; The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.; Authors - Anne Roobol
human	WB,IF,CoIP	Ecotoxicol Environ Saf; Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.; Authors - Yujing Zhang
human	WB	Biochem J; Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.; Authors - Emma Jane Mead
human	WB	Vet Microbiol; EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein.; Authors - Wei Pan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (06-26-2023)

the antibody works well for Western Blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293