EIF3G Polyclonal antibody

EIF3G Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11165-1-AP

Synonyms

eIF-3 RNA-binding subunit, eIF3 p44, eIF3 p42, eIF3 delta, eIF 3 RNA binding subunit

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Validation Data Gallery

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  WB analysis of HepG2 using 11165-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11165-1-AP (EIF3G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11165-1-AP

  Immunofluorescent analysis of HepG2 cells using 11165-1-AP (EIF3G antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 11165-1-AP

  Immunofluorescent analysis of A549 cells, using EIF3G antibody 11165-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue, mouse colon tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

11165-1-AP targets EIF3G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3G fusion protein Ag1653
• Full Name: eukaryotic translation initiation factor 3, subunit G
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000733
• Gene Symbol: EIF3G
• Gene ID (NCBI): 8666
• RRID: AB_2096911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3G is a component of the EIF3 complex. [PMID:20679478,20503360]

Publications

Species	Application	Title
mouse	WB	Nat Chem Biol; Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.; Authors - Xin Erica Shu
human	IF	PLoS Pathog; The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation.; Authors - Jordan M Meyers
human	IF	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas