EIF3E Polyclonal antibody
EIF3E Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 10899-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat lymph tissue, HeLa cells, mouse thymus tissue, mouse pancreas tissue, Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
10899-1-AP targets EIF3E in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EIF3E fusion protein Ag1132 |
Full Name | eukaryotic translation initiation factor 3, subunit E |
Calculated Molecular Weight | 445 aa, 52 kDa |
Observed Molecular Weight | 48-52 kDa |
GenBank Accession Number | BC008419 |
Gene Symbol | EIF3E |
Gene ID (NCBI) | 3646 |
RRID | AB_2096898 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for EIF3E antibody 10899-1-AP | Download protocol |
IHC protocol for EIF3E antibody 10899-1-AP | Download protocol |
IF protocol for EIF3E antibody 10899-1-AP | Download protocol |
IP protocol for EIF3E antibody 10899-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging Cell Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells. | ||
Cancer Genomics Proteomics Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate | ||
Cancer Lett N6-Methyladenosine enhances the translation of ENO1 to promote the progression of bladder cancer by inhibiting PCNA ubiquitination |