DNAJB1 Polyklonaler Antikörper

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• KD/KO Validated

DNAJB1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Rezensionen (1)

Kat-Nr. : 13174-1-AP

Synonyme

Heat shock 40 kDa protein 1, hDj-1, Hdj1, hDj 1, DNAJ1

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  WB analysis using 13174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13174-1-AP

  IP result of anti-DNAJB1 (IP:13174-1-AP, 4ug; Detection:13174-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse testis using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13174-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (13174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 13174-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (13174-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, C6-Zellen, Mauslebergewebe, Mauslungengewebe, Rattenlebergewebe, Rattenlungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Lymphomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13174-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA DNAJB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB1 fusion protein Ag3830
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 1
• Berechnetes Molekulargewicht: 340 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC019827
• Gene symbol: DNAJB1
• Gene ID (NCBI): 3337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Caspase-2 is a condensate-mediated deubiquitinase in protein quality control; Authors - Yingwei Ge
rat	IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
human	WB	Int J Mol Sci; PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.; Authors - Yanqing Li
	WB	Front Oncol; A Prognostic Prediction Model Developed Based on Four CpG Sites and Weighted Correlation Network Analysis Identified DNAJB1 as a Novel Biomarker for Pancreatic Cancer.; Authors - Lingming Kong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ana (Verified Customer) (03-11-2021)

DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Liver & Heart

DNAJB1 Antibody Western Blot validation (1:1000 dilution) in Mouse Liver & Heart (Cat no:13174-1-AP)