DMAP1 Polyklonaler Antikörper

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• KD/KO Validated

DMAP1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10411-1-AP

Synonyme

EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1

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Galerie der Validierungsdaten

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  WB analysis using 10411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10411-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10411-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10411-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 10411-1-AP

  IP result of anti-DMAP1 (IP:10411-1-AP, 4ug; Detection:10411-1-AP 1:1000) with COLO 320 cells lysate 2320 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10411-1-AP bindet in WB, IP, ChIP, RIP, ELISA DMAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DMAP1 fusion protein Ag0665
• Vollständiger Name: DNA methyltransferase 1 associated protein 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53-60 kDa
• GenBank-Zugangsnummer: BC002855
• Gene symbol: DMAP1
• Gene ID (NCBI): 55929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2). The antibody reacts specifically with DMAP1 protein.

Publikationen

Species	Application	Title
human	WB	Cell; An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.; Authors - Thomas G Fazzio ; KD Validated
mouse		Nat Struct Mol Biol; R loops regulate promoter-proximal chromatin architecture and cellular differentiation.; Authors - Poshen B Chen
human	WB	EMBO Mol Med; Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis.; Authors - Jinbeom Heo
mouse	WB	Elife; Hdac6 regulates Tip60-p400 function in stem cells.; Authors - Chen Poshen B PB
mouse	WB	Cell Rep; Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l.; Authors - Jinbeom Heo
mouse	ChIP,RIP	Psychiatry Investig; m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis; Authors - Yue Ming

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