DNMT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

82

Reviews (1)

Cat no : 24206-1-AP

Synonyms

AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, MCMT

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Validation Data Gallery

• 

  WB analysis using 24206-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 24206-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24206-1-AP

  IP result of anti-DNMT1 (IP:24206-1-AP, 4ug; Detection:24206-1-AP 1:500) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human lung cancer using 24206-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 24206-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNMT1 antibody (24206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 76 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Product Information

24206-1-AP targets DNMT1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNMT1 fusion protein Ag19116
• Full Name: DNA (cytosine-5-)-methyltransferase 1
• Calculated Molecular Weight: 1632 aa, 185 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC126227
• Gene Symbol: DNMT1
• Gene ID (NCBI): 1786
• RRID: AB_2879457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)

Publications

Species	Application	Title
human	WB	Cell Death Dis; Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis; Authors - Bowen Liu
zebrafish	WB	Chemosphere; Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae; Authors - Fang Jiao
human	WB,IF,ChIP	Bioact Mater; Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.; Authors - Jin Wang ; KO Validated
human	CoIP	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	J Transl Med; The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy; Authors - Liangliang Zhao
human	WB	Respir Res; Oxidative stress mediates the apoptosis and epigenetic modification of the Bcl-2 promoter via DNMT1 in a cigarette smoke-induced emphysema model.; Authors - Huihui Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paula (Verified Customer) (12-14-2021)

good bands at the right molecular weight, but a lot of smear

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: fetal rat lung tissue

DNMT1 Antibody Western Blot validation (1:500 dilution) in fetal rat lung tissue (Cat no:24206-1-AP)

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