• Featured Product
  • KD/KO Validated

TSG101 Polyclonal antibody

TSG101 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 14497-1-AP


ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14497-1-AP targets TSG101 in WB, IP, IF, FC, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSG101 fusion protein Ag5920
Full Name tumor susceptibility gene 101
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.


Product Specific Protocols
WB protocol for TSG101 antibody 14497-1-APDownload protocol
IHC protocol for TSG101 antibody 14497-1-APDownload protocol
IF protocol for TSG101 antibody 14497-1-APDownload protocol
IP protocol for TSG101 antibody 14497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


human, mouseWB, IHC


Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.

Authors - Lin Zhang


Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.

Authors - Mirjam C Boelens


Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.

Authors - Barzin Y Nabet

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
  • KD Validated

ACS Nano

Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.

Authors - Meng Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Pasquale (Verified Customer) (11-06-2019)

The antibody recognize the exosomal marker TSG101 as claimed. We tested it by Western blotting in our exosome preps isolated from murine brains and it gave the signal at the correct molecular weight in the fractions that are enriched in exosomes, so it seems to be specific. However, we report here some drawbacks that we have experienced using this antibody (see the file attached):1. There is a strong cross-reactivity of the antibody with the molecular weight marker we use (The Bio-Rad Dual Color Standard, cat. number 1610374)2. The signal over noise ratio is a little low (the background coming from the membrane is pretty high)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Murine brain extracellular vesicles fractions
TSG101 Antibody Western Blot, validation (1 to 1000 dilution) in Murine brain extracellular vesicles fractions (Cat no:14497-1-AP)