CREB3L1,OASIS Polyklonaler Antikörper

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• KD/KO Validated

CREB3L1,OASIS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 11235-2-AP

Synonyme

CREB3L1, OASIS, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the maturer form likely due to cell stage.

• 

  WB analysis of COLO 320 using 11235-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the precursor form likely due to cell stage.

• 

  IHC staining of human prostate cancer using 11235-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of A549 using 11235-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 4 publications below

Produktinformation

11235-2-AP bindet in WB, IHC, IF/ICC, ELISA CREB3L1,OASIS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREB3L1,OASIS fusion protein Ag1757
• Vollständiger Name: cAMP responsive element binding protein 3-like 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa and 84 kDa
• GenBank-Zugangsnummer: BC015781
• Gene symbol: CREB3L1/OASIS
• Gene ID (NCBI): 90993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

Publikationen

Species	Application	Title
	WB	Epigenetics; OASIS/CREB3L1 is epigenetically silenced in human bladder cancer facilitating tumor cell spreading and migration in vitro.; Authors - Michael Rose
rat	WB	Endocrinology; OASIS/CREB3L1 induces expression of genes involved in extracellular matrix production but not classical endoplasmic reticulum stress response genes in pancreatic beta-cells.; Authors - Vellanki Ravi N RN
human	WB,IHC	Breast Cancer Res; Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers.; Authors - Alison K Ward
human, rat	WB	PLoS One; OASIS/CREB3L1 is induced by endoplasmic reticulum stress in human glioma cell lines and contributes to the unfolded protein response, extracellular matrix production and cell migration.; Authors - Vellanki Ravi N RN ; KD Validated
human	WB,IHC	BMC Cancer; CREB3L1 as a potential biomarker predicting response of triple negative breast cancer to doxorubicin-based chemotherapy.; Authors - Bray Denard
rat	WB	Front Endocrinol (Lausanne); Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.; Authors - Xiaomei Liu

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