NFE2L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NFE2L1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 12936-1-AP

Synonyms

HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1

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Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 12936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12936-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12936-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12936-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12936-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12936-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Fetal mouse bone using 12936-1-AP

  Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Fetal mouse bone using 12936-1-AP

  Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12936-1-AP

  IP result of anti-NFE2L1 (IP:12936-1-AP, 4ug; Detection:12936-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of human breast cancer using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, Fetal mouse bone tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12936-1-AP targets NFE2L1 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFE2L1 fusion protein Ag3916
• Full Name: nuclear factor (erythroid-derived 2)-like 1
• Calculated Molecular Weight: 772 aa, 85 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC010623
• Gene Symbol: NFE2L1
• Gene ID (NCBI): 4779
• RRID: AB_2267298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against a region of human NFE2L1.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
mouse	WB,IP	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang
mouse	WB	Redox Biol; Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression.; Authors - Peng Xue
mouse	WB	Free Radic Biol Med; Novel mechanistic insight on the neuroprotective effect of berberine: The role of PPARδ for antioxidant action.; Authors - Jia-Wen Shou
human	WB	Ecotoxicol Environ Saf; Long-isoform NFE2L1 silencing inhibits acquisition of malignant phenotypes induced by arsenite in human bronchial epithelial cells.; Authors - Yongfang Li ; KO Validated
mouse	WB	Food Chem Toxicol; Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice; Authors - Simeng Bao