FUS/TLS Polyclonal antibody

• Featured Product
• KD/KO Validated

FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

108

Reviews (6)

Cat no : 11570-1-AP

Synonyms

75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 11570-1-AP

  WB result of FUS antibody (11570-1-AP, 1:5000) with si-Control and si-FUS transfected HEK 293 cells.

• 

  WB analysis of HeLa using 11570-1-AP

  HeLa (WT and FUS KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 11570-1-AP incubated at 1:5000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 11570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11570-1-AP

  IP result of anti-FUS/TLS(IP:11570-1-AP, 4ug; Detection:11570-1-AP 1:2000) with K-562 cells lysate 1760 ug.

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 11570-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11570-1-AP (FUS/TLS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11570-1-AP

  HeLa WT cells (yellow outline) and FUS KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11570-1-AP at 1:2000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IF Staining of HeLa using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUS/TLS antibody (11570-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11570-1-AP

  1X10^6 HeLa cells were stained with 0.2ug FUS/TLS antibody (11570-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, human ovary tumor tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 56 publications below
• IHC: See 32 publications below
• IF: See 42 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 4 publications below

Product Information

11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUS/TLS fusion protein Ag2150
• Full Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Calculated molecular weight: 75 kDa
• Observed molecular weight: 68-75 kDa
• GenBank accession number: BC026062
• Gene symbol: FUS
• Gene ID (NCBI): 2521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. FUS was detected double bands of 68-74 kDa (PMID:31519807).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC,IF	Nature; Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.; Authors - Deng Han-Xiang HX
human,mouse	WB	Nat Med; Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.; Authors - Vladislav A Korobeynikov
human	WB,IF	Cell; Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.; Authors - Lin Guo
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.; Authors - Gina Picchiarelli ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

manohar (Verified Customer) (07-10-2023)

Nitrocellulose membrane is used with 5% milk as blocking and antibody diluted in 1% milk and incubated overnight.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Tatyana (Verified Customer) (05-14-2023)

ICC using 5% goat serum/PBST buffer, 2 hours at RT. Good specific nuclear signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

FUS/TLS Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11570-1-AP)

FH

shashirekha (Verified Customer) (12-23-2020)

Used for immunopreciptation at 1:1000 dilution. Works very well

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat cortical neurons

FUS/TLS Antibody Immunoprecipitation validation (1:1000 dilution) in Rat cortical neurons (Cat no:11570-1-AP)

FH

H (Verified Customer) (04-06-2020)

The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

FUS/TLS Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:11570-1-AP)

FH

Karthik (Verified Customer) (04-24-2019)

Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 overnight at 4 degrees
• Cell Tissue Type: motor neurons

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human motor neuron

Related Products

CHOP; GADD153 Polyclonal antibody

15204-1-AP

TDP-43 Polyclonal antibody

10782-2-AP

EWS Polyclonal antibody

55191-1-AP

TAF15 Polyclonal antibody

24235-1-AP

ERG Polyclonal antibody

14356-1-AP