CREB3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CREB3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 11275-1-AP

Synonyms

Luman, Leucine zipper protein, Cyclic AMP-responsive element-binding protein 3, CREB-3, CREB 3

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Validation Data Gallery

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  WB analysis of Jurkat using 11275-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11275-1-AP (CREB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11275-1-AP

  WB result of CREB3 antibody (11275-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB3 transfected HeLa cells.

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  IHC staining of human prostate cancer using 11275-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11275-1-AP (CREB3 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of U2OS using 11275-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CREB3 antibody (11275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• ChIP: See 3 publications below

Product Information

11275-1-AP targets CREB3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CREB3 fusion protein Ag1823
• Full Name: cAMP responsive element binding protein 3
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40-64 kDa
• GenBank Accession Number: BC009402
• Gene Symbol: CREB3
• Gene ID (NCBI): 10488
• RRID: AB_10640533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

Publications

Species	Application	Title
human	WB	Sci Adv; Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing.; Authors - Tejabhiram Yadavalli ; KD Validated
human	ChIP	Nat Commun; Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation.; Authors - Miao Wang
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB,IHC	Cancers (Basel); miR766-3p and miR124-3p Dictate Drug Resistance and Clinical Outcome in HNSCC; Authors - Tomohiro Shibata
human	WB,IF,IP,ChIP	Front Mol Neurosci; LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion.; Authors - Jenna Penney
human		Carcinogenesis; Small leucine zipper protein promotes the metastasis of castration-resistant prostate cancer through transcriptional regulation of matrix metalloproteinase-13.; Authors - Suhyun Kim