COTL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COTL1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 10781-1-AP

Synonyme

CLP, Coactosin like protein, COTL1

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Galerie der Validierungsdaten

• 

  WB analysis using 10781-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10781-1-AP

  WB result of COTL1 antibody (10781-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells.

• 

  IP experiment of mouse kidney using 10781-1-AP

  IP result of anti-COTL1 (IP:10781-1-AP, 3ug; Detection:10781-1-AP 1:3000) with mouse kidney tissue lysate 12500ug.

• 

  IHC staining of human lung cancer using 10781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP (COTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP (COTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

10781-1-AP bindet in WB, IHC, IF, IP, ELISA COTL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COTL1 fusion protein Ag1230
• Vollständiger Name: coactosin-like 1 (Dictyostelium)
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC010884
• Gene symbol: COTL1
• Gene ID (NCBI): 23406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Publikationen

Species	Application	Title
human	WB,IHC	J Proteome Res; Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.; Authors - Jeong Hye-Cheol HC
human	WB	Oncotarget; Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.; Authors - Shanqi Guo
human	IHC	J Inflamm Res; Coactosin-Like Protein in Breast Carcinoma: Friend or Foe?; Authors - Bei Wang
human	WB	PLoS One; TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.; Authors - Somalinga Balajee R BR ; KD Validated
human	WB	Exp Lung Res; Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.; Authors - Claudia D'Anna
human	WB	J Investig Med; Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.; Authors - Sun Wenjing W

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Susanne (Verified Customer) (06-29-2019)

Good staining with low background in WB and IF.For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS.For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 WB 1:50 IF
• Cell Tissue Type: EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells

COTL1 Antibody Western Blot,Immunofluorescence, validation (1:1000 WB 1:50 IF dilution) in EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells (Cat no:10781-1-AP)

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