CLTB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CLTB Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10455-1-AP

Synonyme

LCB, clathrin, light chain (Lcb), Clathrin light chain B

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse thymus using 10455-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10455-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10455-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10455-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10455-1-AP

  IP result of anti-CLTB (IP:10455-1-AP, 4ug; Detection:10455-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of HeLa using 10455-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLTB antibody (10455-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10455-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CLTB antibody (10455-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, humanes Hirngewebe, Maushirngewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

10455-1-AP bindet in WB, IF/ICC, IP, ELISA CLTB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLTB fusion protein Ag0707
• Vollständiger Name: clathrin, light chain (Lcb)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 32-38 kDa
• GenBank-Zugangsnummer: BC006457
• Gene symbol: CLTB
• Gene ID (NCBI): 1212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Clathrin light chains' role in selective endocytosis influences antibody isotype switching.; Authors - Shuang Wu ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.; Authors - Lisa Redlingshöfer
human	WB	J Virol; Dabie bandavirus Nonstructural Protein Interacts with Actin to Induce F-Actin Rearrangement and Inhibit Viral Adsorption and Entry.; Authors - Hongyun Liu
human	IF	bioRxiv; Downregulation of lysosomal trafficking in ARPE19 cells leads to decreased transfection efficiency at high passage; Authors - Erika M S Hood

Verwandte Produkte

CLTA Polyclonal antibody

10852-1-AP

HIP1R Polyclonal antibody

16814-1-AP

CLTC Polyclonal antibody

26523-1-AP

AP2B1 Polyclonal antibody

15690-1-AP

CLTCL1 Polyclonal antibody

22283-1-AP

CLTCL1 Monoclonal antibody

66274-1-Ig

HIP1-Specific Polyclonal antibody

19688-1-AP