CLTA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CLTA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (3)

Kat-Nr. : 10852-1-AP

Synonyme

LCA, clathrin, light chain (Lca), Clathrin light chain A

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Galerie der Validierungsdaten

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  WB analysis using 10852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10852-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10852-1-AP

  IP result of anti-CLTA (IP:10852-1-AP, 4ug; Detection:10852-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10852-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10852-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CLTA antibody (10852-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10852-1-AP (CLTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C2C12-Zellen, C6-Zellen, K-562-Zellen, Maushirngewebe, PC-12-Zellen, Raji-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 12 publications below
• IP: See 1 publications below

Produktinformation

10852-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CLTA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLTA fusion protein Ag1299
• Vollständiger Name: clathrin, light chain (Lca)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC019287
• Gene symbol: CLTA
• Gene ID (NCBI): 1211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.; Authors - Tomasz J Nawara
human	WB	Proc Natl Acad Sci U S A; Clathrin light chains' role in selective endocytosis influences antibody isotype switching.; Authors - Shuang Wu ; KO Validated
human	WB	J Cell Biol; Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.; Authors - Gilles Moulay
		Small; Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.; Authors - Ji-Qian Zhang
rat	IF	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
rat	IHC	J Bone Miner Res; Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis.; Authors - Lacruz Rodrigo S RS

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

K (Verified Customer) (06-10-2021)

this Ab works well for western blotting in mouse, human and rat cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000

FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

CLTA Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10852-1-AP)

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