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CLIC3 Polyklonaler Antikörper

CLIC3 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15971-1-AP

Synonyme

CLIC3

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using CLIC3 Polyclonal antibody (15971-1-AP)

    WB analysis using 15971-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15971-1-AP (CLIC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CLIC3 Polyclonal antibody (15971-1-AP)

    IHC staining of human placenta using 15971-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CLIC3 Polyclonal antibody (15971-1-AP)

    IHC staining of human placenta using 15971-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CLIC3 Polyclonal antibody (15971-1-AP)

    IF Staining of MCF-7 using 15971-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CLIC3 antibody 15971-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJAR-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe, Rattennierengewebe
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below
IHCSee 1 publications below

Produktinformation

15971-1-AP bindet in WB, IHC, IF, ELISA CLIC3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CLIC3 fusion protein Ag8757
Vollständiger Name chloride intracellular channel 3
Berechnetes Molekulargewicht 236 aa, 26 kDa
Beobachtetes Molekulargewicht 27-30 kDa
GenBank-ZugangsnummerBC007012
Gene symbol CLIC3
Gene ID (NCBI) 9022
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.

Protokolle

Produktspezifische Protokolle
WB protocol for CLIC3 antibody 15971-1-APProtokoll herunterladen
IHC protocol for CLIC3 antibody 15971-1-APProtokoll herunterladen
IF protocol for CLIC3 antibody 15971-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIHC

PLoS One

Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.

Authors - Liddelow Shane A SA
View Article
humanWB

J Cancer

Analysis of Differentially Expressed Genes in a Chinese Cohort of Esophageal Squamous Cell Carcinoma.

Authors - Gang Liu
View Article
human,ratWB

Front Cell Neurosci

Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms

Authors - Xiaoli Zhu
View Article