CLIC1 Polyclonal antibody

• 

  WB analysis of HL-60 using 14545-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14545-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14545-1-AP

  IP result of anti-CLIC1 (IP:14545-1-AP, 4ug; Detection:14545-1-AP 1:300) with HeLa cells lysate 1200ug.

• 

  IHC staining of mouse colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14545-1-AP (CLIC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14545-1-AP (CLIC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14545-1-AP

  Immunofluorescent analysis of HepG2 cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14545-1-AP

  Immunofluorescent analysis of Hela cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• Featured Product
• KD/KO Validated

CLIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14545-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

G6, NCC27

---

Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse colon tissue, human colon tissue, human pancreas tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

14545-1-AP targets CLIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLIC1 fusion protein Ag6049
• Full Name: chloride intracellular channel 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-32 kDa
• GenBank Accession Number: BC064527
• Gene Symbol: CLIC1
• Gene ID (NCBI): 1192
• RRID: AB_2081809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00299
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).

Publications

Species	Application	Title
		Nat Commun; CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.; Authors - Tiantian Tang
mouse	WB	Alzheimers Res Ther; Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis; Authors - Deng-Pan Wu
rat	IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li
human	WB	Transl Oncol; Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling; Authors - Huiwu Geng ; KD Validated
		Cell Biochem Biophys; CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.; Authors - Dezhao Lu
human	IHC	Anticancer Res; Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.; Authors - Yuichiro Yasuda