CHMP4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHMP4B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

53

Rezensionen (1)

Kat-Nr. : 13683-1-AP

Synonyme

SHAX1, hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B

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Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 13683-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13683-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13683-1-AP (CHMP4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13683-1-AP (CHMP4B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, C2C12-Zellen, H9C2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 19 publications below
• IF: See 37 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

13683-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CHMP4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHMP4B fusion protein Ag4544
• Vollständiger Name: chromatin modifying protein 4B
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-33 kDa
• GenBank-Zugangsnummer: BC033859
• Gene symbol: CHMP4B
• Gene ID (NCBI): 128866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Publikationen

Species	Application	Title
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
		Neuron; Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.; Authors - Juliet Goldsmith
human	CoIP	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	IF	Sci Adv; Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.; Authors - Virginia Andrade

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sharad (Verified Customer) (08-30-2019)

The antibody works well in IF staining 1:500 dilution.

• Applications: Immunofluorescence,
• Cell Tissue Type: RPE-1

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