CHMP2B Polyklonaler Antikörper

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• KD/KO Validated

CHMP2B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 12527-1-AP

Synonyme

hVps2 2, DMT1, Chromatin-modifying protein 2b, chromatin modifying protein 2B, CHMP2.5

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 12527-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human ileum using 12527-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12527-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12527-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12527-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12527-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12527-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12527-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12527-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PFA fixed cells using 12527-1-AP

  IF result (endosomal stain) of anti-CHMP2B (12527-1-AP) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman.

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  IF Staining of HepG2 using 12527-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Ileumgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, PFA-fixierte Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

12527-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CHMP2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHMP2B fusion protein Ag3222
• Vollständiger Name: chromatin modifying protein 2B
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC001553
• Gene symbol: CHMP2B
• Gene ID (NCBI): 25978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
pig	WB,IF,IP	PLoS Pathog; Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.; Authors - Chun-Chun Liu
human	IF	Cell Death Dis; iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.; Authors - Kun Gao
mouse	WB	J Neurosci; Nascent Aβ42 Fibrillization in Synaptic Endosomes Precedes Plaque Formation in a Mouse Model of Alzheimer's-like β-Amyloidosis; Authors - Elizabeth A Eckman
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo
mouse	IF	Sci Rep; VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.; Authors - Carolyn Ott

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