VPS4A Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14272-1-AP

Synonyms

hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A

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Validation Data Gallery

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  WB analysis using 14272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14272-1-AP

  WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells.

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  WB analysis using 14272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 14272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14272-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 14272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14272-1-AP targets VPS4A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS4A fusion protein Ag5592
• Full Name: vacuolar protein sorting 4 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC047932
• Gene Symbol: VPS4A
• Gene ID (NCBI): 27183
• RRID: AB_2878039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
canine	WB	Parasitol Res; Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum.; Authors - Francesca De Falco
human	WB	Theranostics; Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy; Authors - Kunjian Lei