Chromogranin A Polyklonaler Antikörper

Chromogranin A Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (4)

Kat-Nr. : 10529-1-AP

Synonyme

CHGA, CGA, ER-37, EA-92, Chromogranin-A

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Galerie der Validierungsdaten

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  WB analysis of PC-12 using 10529-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10529-1-AP (Chromogranin A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10529-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10529-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human pancreas using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10529-1-AP (Chromogranin A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10529-1-AP (Chromogranin A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human pancreas using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10529-1-AP (Chromogranin A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10529-1-AP (Chromogranin A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse pancreas using 10529-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 10529-1-AP (Chromogranin A antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 10529-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (10529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolongewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe, Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

10529-1-AP bindet in WB, IHC, IF-P, ELISA Chromogranin A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag0807
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: Chromogranin A
• Gene ID (NCBI): 1113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publikationen

Species	Application	Title
mouse	IF	Cell Metab; Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance.; Authors - Yanru Wu
human	WB	Cell Biosci; Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer; Authors - Qiong Wang
human	WB	Sci Rep; Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line.; Authors - Azita Monazzam
human	WB	Discov Oncol; Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma; Authors - Hao Liu
mouse	IHC	Dose Response; The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.; Authors - Danjie Liu
human	WB	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

balawant (Verified Customer) (07-26-2022)

This Antibody is working great. I have used for colon and colon cancer cell line

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Susmita (Verified Customer) (04-13-2022)

This antibody works excellent.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 1500
• Cell Tissue Type: PC cell line

FH

James (Verified Customer) (03-31-2021)

Antibody worked well in both recombinant Chromogranin A produced in both HeLa cell lysates and in HEK cells overexpressed with Chromogranin A

• Applications: Western Blot, Cell culture
• Cell Tissue Type: HeLa cell lysate IVT expression and HEK cell overexpression

FH

Iram (Verified Customer) (08-14-2020)

Used 1:1000 dilution for immunoblotting. It gives a very clean band.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer

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