Cadherin-16 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cadherin-16 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15107-1-AP

Synonyme

cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin

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Galerie der Validierungsdaten

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  WB analysis using 15107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15107-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15107-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15107-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293 using 15107-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug Cadherin-16 antibody (15107-1-AP, red) and control antibody (blue). Fixed with blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, humanes Nierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 4 publications below

Produktinformation

15107-1-AP bindet in WB, IHC, IF, ELISA Cadherin-16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cadherin-16 fusion protein Ag4343
• Vollständiger Name: cadherin 16, KSP-cadherin
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC027912
• Gene symbol: CDH16
• Gene ID (NCBI): 1014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

Publikationen

Species	Application	Title
mouse	IF	Int Immunopharmacol; Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion; Authors - Zhi Zhao
mouse	WB,IF	Front Cell Dev Biol; Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition.; Authors - Yanhong Zhang
mouse	WB,IF	J Cell Sci; Pax8 controls thyroid follicular polarity through Cadherin-16.; Authors - Petrina Koumarianou ; KD Validated
human	WB	J Cancer; TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers.; Authors - Jiaqi Dai
human	WB	Oncol Lett; Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.; Authors - Xiaolin Yang
mouse	IF	Cell Death Dis; Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis.; Authors - Huzi Xu

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