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BST2 Polyklonaler Antikörper
BST2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF/ICC, IF-P, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13560-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, L02-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | Maus-Augengewebe, NCCIT-Zellen |
| Erfolgreiche Detektion in IF/ICC | NCCIT-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 8 publications below |
| WB | See 25 publications below |
| IHC | See 6 publications below |
| IF | See 10 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Produktinformation
13560-1-AP bindet in WB, IHC, IF/ICC, IF-P, CoIP, ELISA BST2 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | BST2 fusion protein Ag4430 |
| Vollständiger Name | bone marrow stromal cell antigen 2 |
| Berechnetes Molekulargewicht | 180 aa, 20 kDa |
| Beobachtetes Molekulargewicht | 30-36 kDa, 50-70 kDa |
| GenBank-Zugangsnummer | BC033873 |
| Gene symbol | BST2 |
| Gene ID (NCBI) | 684 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks retrovirus release from the cell surface. Depleted upon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane protein, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 are 30-36 kDa (monomer form) and 50-70 kDa (dimer form) (PMID: 19196977; 21237475; 19737401; 37922253).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for BST2 antibody 13560-1-AP | Protokoll herunterladen |
| IHC protocol for BST2 antibody 13560-1-AP | Protokoll herunterladen |
| IF protocol for BST2 antibody 13560-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Curr Biol The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.
| ||
J Virol Interferon-inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion.
| ||
J Virol Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Margaux (Verified Customer) (06-13-2024) | Very good efficiency. In HeLa cells, I detected BST2 dimers and monomers at the dilution 1/1000.
 |
FH Christine (Verified Customer) (10-08-2021) | Detects endogenous BST2 in HeLa cells (BST2 positive), also when overexpressed as FLAG tagged version in HeLa cells. Does no detect anything in HEK293T cells (BST2 negative) but detects many bands when FLAG BST2 or untagged BST2 is overexpressed in these cells.
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