HGS Polyclonal antibody

• Featured Product
• KD/KO Validated

HGS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10390-1-AP

Synonyms

ZFYVE8, Protein pp110, Hrs, Hepatocyte growth factor-regulated tyrosine kinase substrate

---

Validation Data Gallery

• 

  WB analysis using 10390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10390-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10390-1-AP

  IP result of anti-HGS (IP:10390-1-AP, 5ug; Detection:10390-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

• 

  IHC staining of human liver using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10390-1-AP (HGS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10390-1-AP (HGS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10390-1-AP (HGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10390-1-AP (HGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10390-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HGS antibody (10390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

10390-1-AP targets HGS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HGS fusion protein Ag0589
• Full Name: hepatocyte growth factor-regulated tyrosine kinase substrate
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC003565
• Gene Symbol: HGS
• Gene ID (NCBI): 9146
• RRID: AB_2118914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IHC	Sci Rep; β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development.; Authors - Yue-Ming Ling
human	WB,IF	Mol Biol Cell; The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.; Authors - Justine Holleman
human	IF	J Cell Sci; Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.; Authors - Dolma Choezom
human	IF	iScience; VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.; Authors - Xiaohua Dong
human	IF	iScience; Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide.; Authors - Asako Goto

Related Products

TSG101 Polyclonal antibody

14497-1-AP