APOBEC3G Monoclonal antibody

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• KD/KO Validated

APOBEC3G Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

5G10A8

Cat no : 60100-1-Ig

Synonyms

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

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Validation Data Gallery

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  WB analysis of PC-3 cells using 60100-1-Ig

  WB result of APOBEC3G antibody (60100-1-Ig, 1:1000) with si-Control and si-APOBEC3G transfected PC-3 cells.

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  WB analysis of A375 using 60100-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 60100-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60100-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60100-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of C6 using 60100-1-Ig

  Immunofluorescent analysis of C6 cells using 60100-1-Ig (APOBEC3G antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Tested Applications

• Positive WB detected in: A375 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

60100-1-Ig targets APOBEC3G in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOBEC3G fusion protein Ag7770
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC024268
• Gene Symbol: APOBEC3G
• Gene ID (NCBI): 60489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Rep; Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.; Authors - Ruidong Chen
human	IHC	Int J Clin Exp Med; APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.; Authors - Huanrong Lan