BMI1 Polyklonaler Antikörper

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• KD/KO Validated

BMI1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Kat-Nr. : 10832-1-AP

Synonyme

RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10832-1-AP

  WB results of BMI antibody (10832-1-AP, 1:20000) with si-Control and si-BMI transfected HEK293 cells..

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  WB analysis of U-937 using 10832-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10832-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10832-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10832-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10832-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 10832-1-AP

  IP result of anti-BMI1 (IP:10832-1-AP, 4ug; Detection:10832-1-AP 1:500) with U-937 cells lysate 4000ug.

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  IHC staining of human liver cancer using 10832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10832-1-AP

  Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 10832-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL34 antibody 10832-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, HEK-293-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 34 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

10832-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA BMI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMI1 fusion protein Ag1286
• Vollständiger Name: BMI1 polycomb ring finger oncogene
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-43 kDa
• GenBank-Zugangsnummer: BC011652
• Gene symbol: BMI1
• Gene ID (NCBI): 648
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Publikationen

Species	Application	Title
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran ; KD Validated
human	WB	Oncogene; Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.; Authors - Yang Chen
mouse	WB,CoIP,ChIP	J Neurosci; Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.; Authors - Kim Mee-Ohk MO
human	WB	Int J Cancer; SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.; Authors - S-L Xie
human	WB	Oncotarget; Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.; Authors - Xiaolong Wei

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