RNF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 16031-1-AP

Synonyms

HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1

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Validation Data Gallery

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  WB analysis of K-562 using 16031-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16031-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16031-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 16031-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16031-1-AP

  IP result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug.

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  IF Staining of HeLa using 16031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, human brain tissue, NIH/3T3 cells, U-937 cells
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

16031-1-AP targets RNF2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF2 fusion protein Ag8900
• Full Name: ring finger protein 2
• Calculated Molecular Weight: 336 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC012583
• Gene Symbol: RNF2
• Gene ID (NCBI): 6045
• RRID: AB_2180459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Cell; Coordinated regulation of polycomb group complexes through microRNAs in cancer.; Authors - Qi Cao ; KD Validated
human	IHC	Cancer Cell; The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.; Authors - Wenjing Su ; KD Validated
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB,IF	J Cell Biol; DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response; Authors - Tessa Arends ; KD Validated
human	WB,IHC	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei
human	WB, IF	Front Cell Dev Biol; RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells.; Authors - Qi Yan ; KD Validated