• Featured Product
  • KD/KO Validated

RNF2 Polyclonal antibody

RNF2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16031-1-AP

Synonyms

BAP 1, BAP1, DING, HIP2 interacting protein 3, HIPI3, Protein DinG, RING finger protein 1B, ring finger protein 2, RING finger protein BAP 1, RING1B, RING2, RNF2



Tested Applications

Positive WB detected inK-562 cells, human brain tissue, NIH/3T3 cells, U-937 cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16031-1-AP targets RNF2 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF2 fusion protein Ag8900
Full Name ring finger protein 2
Calculated Molecular Weight 336 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC012583
Gene Symbol RNF2
Gene ID (NCBI) 6045
RRIDAB_2180459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNF2 antibody 16031-1-APDownload protocol
IF protocol for RNF2 antibody 16031-1-APDownload protocol
IP protocol for RNF2 antibody 16031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Coordinated regulation of polycomb group complexes through microRNAs in cancer.

Authors - Qi Cao
  • KD Validated
humanIHC

Cancer Cell

The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.

Authors - Wenjing Su
  • KD Validated

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
humanWB,IHC

FEBS J

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy

Authors - Yan Wei
humanWB, IF

Front Cell Dev Biol

RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells.

Authors - Qi Yan
  • KD Validated
humanWB

Front Oncol

Long non-coding RNA PRR7-AS1 promotes osteosarcoma progression via binding RNF2 to transcriptionally suppress MTUS1

Authors - Gu Chen-Xi
  • KD Validated